The many advantages of bioengineering and its contribution to making clean and cheap bioproduction

The local context must be considered as, for example, mudbrick may not be durable in a high rainfall area although a large roof overhang and cement stabilisation can be used to correct for thisand, if the materials are not readily available, the method may be inappropriate.

The many advantages of bioengineering and its contribution to making clean and cheap bioproduction

Genetically engineering a common fungus to produce biodiesel from cellulosic waste.

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Converting food into biofuel is an unsustainable proposition. Our project focuses on the creation of cellulosic biodiesel using waste products. We are engineering Neurospora crassa, a highly efficient cellulose metabolizer, to produce an excess of fatty acids by both inhibiting beta oxidation and up-regulating fatty acid synthesis by the one-step replacement of the FadD gene with a thioesterase gene.

We are testing the growth of Neurospora on a variety of waste substances and are developing an efficient chemical esterification method to convert the fatty acids into fatty acid methyl esters, a common biodiesel requiring no changes to current fuel delivery infrastructure.

Neurospora crassa's broad substrate preferences give it unique advantages for bioproduction from cellulose. We have therefore developed an efficient and reliable system for modular bioengineering of Neurospora including a starter kit of basic reusable parts with the intent of creating a novel chassis for metabolic engineering and synthetic biology.

The many advantages of bioengineering and its contribution to making clean and cheap bioproduction

CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.

Making Synthetic Biology More Accessible: Making Wetware and Dryware for the Synthetic Biology Lab The overall mission of our team is to attempt to overcome some practical barriers to entry of groups and laboratories that may not be well-funded or may not have the capital requirements to realize their synthetic biology dreams.

We approach this problem from two angles- "wet-ware" and "dry-ware. Our dry-ware goal this year is to create a prototype for a PCR machine that can be assembled from an inexpensive kit.

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One strain of E. Another strain of E. Depending on the direction of flow, one group of E. Acting as an analog of zener diode, this device is capable of controlling the direction of communication between the E. The orthogonal visual responses, one fluorescent and one luminescent, should be clear indicators of which way communication took place.

This device is a novel member of the growing toolkit available to perform logic operations with living systems. Vibrio cholerae's ToxR system can be used as a component in biological devices capable of detecting a wide variety of molecules.

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A periplasmic domain causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization and gene expression to the presence of specific ligands.

Initially, ToxR constructs proved to be toxic to E. To address ToxR toxicity, we screened microarray data for promoters that exhibited stress-based down regulation.The Wood–Werkman pathway is the metabolic pathway that is most commonly used by PAB and the oxidation state of the substrate is another important factor in determining whether production of both or one of the acids is favored.

Environmental Bio-electrochemistry Interested in the mechanism of extracellular electron transfer and its manipulation, microbial fuel cell and its energy harvesting technology from organic pollutants, and biodegradation of emerging new pollutants, bio-sensing environmental monitoring.

The brewing, winemaking and distilling industries produce alcohol as a beverage, industrial solvent or fuel. These three processes exhibit strong similarities (fermentation and separation operations) and stand as important water consumers and wastewater producers.

October 10th, marked the 9th anniversary for the Journal of Biological Engineering (JBE), the official journal of Institute of Biological Engineering (IBE), published by BioMed Central.

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ACS Editors' Choice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. Excimer lasers emitting ultraviolet light with wavelengths shorter than nm are useful in refractive surgery in ophthalmology, which utilizes the non-thermal ablation effect.

Excimer lasers are also frequently applied in gene manipulation and other bioengineering applications using the high photon energy of ultraviolet light.

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